There are several manual methods to separate peripheral blood mononuclear cells (PBMCs) from leukapheresis products, but most of them are highly user‑dependent and laborious. The Gibco™ CTS™ Rotea™ Counterflow Centrifugation System can be used to automate a variety of methods to separate PBMCs in a closed environment.

In this application note, you’ll see effective and consistent PBMC separation from the CTS Rotea system across all tested methods compared to a manual density gradient centrifugation method.

Test methods include:

• Ficoll™ solution
• With ACK (ammonium-chloride-potassium) lysis buffer
• Without lysis buffer